Replicative Study in Performance-Related Genes of Brazilian Elite Soccer Players Highlights Genetic Differences from African Ancestry and Similarities between Professional and U20 Youth Athletes.

Classically, genetic association studies have attempted to assess genetic polymorphisms related to human physiology and physical performance. However, the heterogeneity of some findings drives the research to replicate, validate, and confirmation as essential aspects for ensuring their applicability in sports sciences. Genetic distance matrix and molecular variance analyses may offer an alternative approach to comparing athletes' genomes with those from public databases. Thus, we performed a complete sequencing of 44 genomes from male Brazilian first-division soccer players under 20 years of age (U20_BFDSC). The performance-related SNP genotypes were obtained from players and from the "1000 Genomes" database (European, African, American, East Asian, and South Asian). Surprisingly, U20_BFDSC performance-related genotypes had significantly larger FST levels (p < 0.00001) than African populations, although studies using ancestry markers have shown an important similarity between Brazilian and African populations (12-24%). U20_BFDSC were genetically similar to professional athletes, showing the intense genetic selection pressure likely to occur before this maturation stage. Our study highlighted that performance-related genes might undergo selective pressure due to physical performance and environmental, cognitive, and sociocultural factors. This replicative study suggests that molecular variance and Wright's statistics can yield novel conclusions in exercise science.

The relationship between internal and external loads as a tool to monitor physical fitness status of team sport athletes: a systematic review.

The efficiency index (Effindex) combines internal and external loads, and it has been considered a promising tool to evaluate physical fitness status. However, its real applicability and limitations have not been elucidated yet. To examine and discuss the findings from studies that used Effindex as a tool for the evaluation of physical fitness status in team sports. A systematic search was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The databases conferred were PubMed, Scopus, Web of Science, SPORTDiscus, MEDLINE and CINAHL. The articles selected were published up to March 2021. Fourteen articles were included after meeting the inclusion criteria. A wide variety of combinations of external and internal loading parameters to calculate Effindex were found. The pooled sample included 349 male participants (23 ± 3 years). Fifty-nine percent of the sample were soccer players, 20% rugby players, 10% Australian football players, 7% hurling players, and 4% basketball players. Most Effindex calculations used total distance (TD) divided by heart rate derived parameters. However, recent studies have suggested the use of accelerations as the external load parameter. Effindex is a simple and powerful tool for the evaluation of physical fitness status in team sports athletes. The disparity of external and internal loading parameters used to calculate Effindex may affect its sensitivity to detect changes in fitness status in different team sport settings. More studies with indoor team sports and female athletes are warranted.

C-Reactive Protein and Skin Temperature of the lower limbs of Brazilian elite soccer players like load markers following three consecutive games.

The aim of the study was to compare the levels and relative responses of C-Reactive Protein (CRP) and Skin Temperature (Tsk) of the lower limbs (LLs) of elite soccer players following three consecutive games with short intervals (< 4 days). Eleven male professional athletes (age, 29.26 ± 4.52 years) from an Brazil elite team were evaluated, with measurements obtained before (Baseline), and at Post-Games times (24, 48 and 72h). CRP and Tsk levels at 24 h and 48 h Post-Games times were higher than Baseline levels (CRP, p < 0.001, d > 0.9; Tsk, p < 0.04, d > 0.58), additionally, the levels of these variables (CRP vs. Tsk) showed positive correlations (p < 0.05, rs = 0.60-0.88). The relative responses these variables were evaluated (Δ%CRP; Δ%Tsk). The 48h responses of Game 3 are respectively higher than 48 h responses of games 1 and 2, in Δ%CRP (p < 0.03; G3 vs. G1, r = 0.66; G3 vs. G2, r = 0.88) and in Δ%Tsk (p < 0.003; G3 vs. G1, r = 0.88; G3 vs. G2, r = 0.88). The 48 h Post-Game time seems to represent valuable contributions to understanding the development regarding the magnitude and duration of inflammatory processes associated with recovery. Additionally, the positive correlations that were verified allow an increase in the robustness of the analysis of the load of consecutive games through CRP and Tsk of the LLs. Then, these results indicate a physiological stress and a probable cumulative effect of the game loads due to short intervals (< 4 days), which is reinforced by the verified outcome of the Total Distance covered (G3 < G2, p = 0.003; r = 0.88). These findings can contribute to better load control and greater robustness of injury prevention programs.

Relationship between infrared thermography and muscle damage markers in physically active men after plyometric exercise.

Plyometric training has been used in several sports and fitness programs to improve jumping ability and explosive strength, both in individual and team sports. Eccentric muscle actions, such as those performed during plyometric jumps, induce muscle damage and consequently a rise in skin temperature (Tsk). Thus, the purpose of this study is to assess the response of infrared thermography measurement as an indirect marker of muscle damage after a protocol of plyometric jumps in physically active subjects. Therefore, for the aim of this study ten male subjects with no previous experience in plyometric training participated in the research (age 22.5 ± 3.3 years, weight 71.7 ± 11.0 kg, height 171.1 ± 5.3 cm, and fat mass 15.5 ± 4.7%). To assess the muscle damage, countermovement jump (CMJ), creatine kinase (CK), delayed-onset muscle soreness (DOMS) and infrared thermography (IRT) were measured at 24, 48, and 72 h after plyometric exercise. The acute exercise protocol of plyometric jumps induced muscle damage, as shown by the CK and DOMS (24 and 48 h, p < 0.05) but no statistical difference was shown between the moments analyzed in Tsk (warm zone). Nevertheless, when comparing baseline to 48h, a moderate effect was found in the Tsk (warm zone) for anterior right thigh (ES = 1.1) and posterior left thigh (ES = 0.9) and large effect was found for anterior left thigh (ES = 1.4) and posterior right thigh (ES = 1.3). A moderate effect in the Tsk (warm zone) was found for posterior right and left thigh (ES = 0.9 and ES = 1.1, respectively) when comparing baseline to 72h of IRT. These results suggest that a plyometric jumping session alters CK and DOMS, as well as the thigh's skin temperature in an evident way, bringing up a possible relation with markers of muscle damage.

Polymorphism of the angiotensin converting enzyme gene (ACE-I/D) differentiates the aerobic and speed performance of football players.

BACKGROUND: The aim of this study was to evaluate the distribution of ACE-I/D polymorphisms on Brazilian football players performance in aerobic capacity, strength and speed tests. METHODS: The participants in this study were 212 Brazilian first division male football players genotyped in DD, ID or II. Genotyping of DNA from leucocytes was performed using polymerase chain reaction and restriction fragment length polymorphism methods. We evaluated speed using a 30-meter sprint test with speed measured at 10 meters (V10), 20 meters (V20), and 30 meters (V30); muscular strength using counter-movement-jump and squat jump tests; and aerobic endurance using the Yo-Yo endurance test. The athletes were ranked in ascending order according to their performance in each test and divided into quartiles: first quartile (0-25%, weak), second (25-50%, normal), third (50-75%, good), and fourth (75-100%, excellent); these were clustered according to genotype frequency. RESULTS: We identified significant differences in the V20 test values and in the aerobic capacity test. Higher frequencies of the ACE-DD genotype were observed in the excellent performance group in the V20. In the aerobic capacity test, higher frequencies of the ACE-II genotype were observed in excellent and good performance groups. CONCLUSIONS: Players with higher performance in anaerobic and aerobic tests are ACE-DD and ACE-II genotypes, respectively.

Correlation between strength and skin temperature asymmetries in the lower limbs of Brazilian elite soccer players before and after a competitive season.

This study aimed to investigate the correlation between strength and skin temperature (Tsk) asymmetries in the lower limbs of elite soccer players before and after a competitive season. The sample (n = 20) was composed of elite male soccer players. Strength asymmetry and Tsk asymmetry was measured through the countermovement jump (CMJ) test and infrared thermography, respectively. Asymmetries were quantified using the symmetry angle (SA) equation. A strong correlation was found between the SA of rate of force development (RFD) and the SA of Tsk before (baseline) (r = 0.7; r2 = 0.43; p = 0.03) and after (r = 0.6; r2 = 0.42; p = 0.04) the competitive season. Moreover, after the competitive season there were significant decreases in Peak force (p < 0.018), Impulse (p < 0.04), RFD (p = 0.0001) and CMJ height (p = 0.05), and significant increases in Tsk (p < 0.03), C-reactive protein (p = 0.03), Creatine kinase (p = 0.0001) and Cortisol (p = 0.04), even after a 3-day rest interval (no training). These results suggest that the CMJ test can be combined with infrared thermography for monitoring strength asymmetry and contribute to the prevention of muscle injuries.

The effect of post-match resistance training on recovery in female footballers; when is best to train?

OBJECTIVE: This study examined the effects of resistance training (RT) performed at 24 or 48h post-match on recovery in female soccer players. METHODS: In a randomized cross-over design, 10 professional female soccer players undertook matches followed by three conditions: Control (no RT), RT-24h and RT-48h post-match. RT was a high-speed and low-load session, consisting of 3 sets of 6 repetitions of lower-body exercises at 50%1RM. During training, one exercise (half-squat) was performed on a force platform to determine mean and peak forces. Tests were undertaken pre, 24, 48 and 72h post-match including countermovement jump (CMJ), 20m sprint, C-reactive protein (CRP) and delayed onset muscle soreness (DOMS). Two-way repeated-measures ANOVA and Effect size (ES) analyses compared the time-course of recovery. RESULTS: Despite no significant differences between conditions, ES for changes from pre to 72h were larger for CMJ, 10 and 20m sprint time, and DOMS in RT48h (ES=0.38-2.13) than in RT24h (ES=0.08-0.66) and in Control (ES=0.09-0.36). No differences in forces of half-squat exercise existed between conditions (p>0.05; ES=0.05-0.06). CONCLUSION: The trend for suppressed recovery of speed, power and perceptual responses at 72h post-match suggests RT48h is less ideal in female soccer players, particularly during congested micro-cycles.

Muscle Damage-Based Recovery Strategies Can Be Supported by Predictive Capacity of Specific Global Positioning System Accelerometry Parameters Immediately a Post-Soccer Match-Load.

ABSTRACT: da Silva, CD, Machado, G, Fernandes, AA, Teoldo, I, Pimenta, EM, Marins, JCB, and Garcia, ES. Muscle damage-based recovery strategies can be supported by predictive capacity of specific global positioning system accelerometry parameters immediately after soccer match-load. J Strength Cond Res 35(5): 1410-1418, 2021-Soccer match-load can be linked to recovery kinetic markers. However, match variability hinders the magnitude of relationship between parameters of interest. Therefore, we examined the correlation between 21 global positioning system accelerometry (GPS-A) parameters and changes in serum creatine kinase (CK) concentrations, muscle soreness (MS), and perceptive recovery quality (PRQ) assessed at baseline (1 h before) and post (0 minute, 2, 4, and 24 hours) a standardized 90-minute match-simulation in 20 university players. Global positioning system accelerometry (15 Hz) data were tested as manufacturer and configurable thresholds. Four GPS-A parameters showed moderate to very large correlations with CK changes at all time points (average speed [avgSP, r = 0.75 to r = 0.84]; running symmetry foot strikes [RSfst, r = 0.53-0.63]; running series [RunS, r = 0.53-0.61]; and acceleration distance [AccD ≥ 1.5 m·s-2; r = 0.46-0.61]). Sprint count (≥2 m·s-2), AccD (≥2.5 m·s-2) and speed exertion (SpEx) had a moderate to large correlation (r = 0.46-0.56) with CK changes from 2 to 24 hours. Changes in MS at 0 minute had large correlation with avgSP (r = 0.53) and moderate with deceleration distance (≥-2 and ≥-3 m·s-2; r = 0.47, r = 0.48, respectively). The PRQ changes had moderate inverse correlation with avgSP at 0 minute (r = -0.39) and SpEx at 2 h (r = -0.69). Our results suggest that during a simulated soccer protocol with a standard workload, only the avgSP has practical application for predicting CK changes over 24 hours, allowing for a decision-making toward a postgame recovery based on previously known CK cutoff points. Global positioning system accelerometry parameters and subjective variables did not demonstrate relevant correlation.

Neuroinvasive chikungunya in a liver transplant recipient.

The influence of chronic immunosuppression on the course of chikungunya virus (CHIKV) infection in recipients of solid organ transplantation (SOT) is still unsettled. Scarce data suggest that the course of CHIKV infection is generally benign in this population. In addition, the occurrence of severe atypical manifestations associated with CHIKV has not been well documented among SOT recipients. In this report, we describe a 64-year-old male liver transplant recipient who was admitted with fever, headache, arthralgia, left palpebral ptosis, mydriasis, and right hemiparesis. Cranial magnetic resonance imaging did not reveal any alteration suggestive of acute infection. Nevertheless, CHIKV was detected in the cerebrospinal fluid (CSF) with a real-time reverse transcriptase assay. Other PCR assays carried out in CSF were negative for HSV-1 and 2, cytomegalovirus, dengue virus (DENV), and Zika virus (ZIKV). CHIKV viremia was also detected while PCR assays for ZIKV and DENV in the blood were negative. ZIKV viruria was simultaneously present in this case. All neurologic manifestations waned within 2 weeks after the onset. This report shows that chikungunya must be considered in the differential diagnosis of acute neurologic disease in SOT recipients who live in or have recently traveled to endemic areas.

Preliminary Validation of Mirrored Scales for Monitoring Professional Soccer Training Sessions.

We aimed to create a single subjective method to assess both internal training loads and subsequent fatigue. This new training-fatigue (dose-response) scale (TFS) was composed of two similar scales with the same properties, metrics and construction criteria. These two scales were designed to rate the perceived exertion (RPETFS) and perceived fatigue (RPFTFS) in professional soccer players. Twenty-two athletes participated to establish reliability, and 15 participated to establish validity. For reliability, the intraclass correlation coefficient (ICC) and standard error of measurement (SEM) were used. For criterion validity, the Spearman's correlation coefficient and linear regression analyses were applied. Associations between RPETFS and RPFTFS were verified by a chi square test, and a further factorial exploratory analysis was conducted. RPETFS and RPFTFS were found to be reliable (ICC 0.74 and 0.77, SEM 0.30 and 0.30, respectively) and valid. RPETFS was best explained by the internal load of the Banister training impulse (p < 0.001), while RPFTFS was best explained by the internal load of the Stagno training impulse (p < 0.001). An association was found between the scales (RPETFS and RPFTFS) in which training duration had a more substantial impact on these subjective perceptions than did training intensity (p < 0.01). RPETFS and RPFTFS scales are reliable and valid for monitoring training sessions in Brazilian professional soccer players. The simultaneous oscillations of the RPETFS and RPFTFS scores can be used by staff members to better plan weekly training programs based on dose-response ratings. Finally, training duration must be carefully controlled because it has a greater impact than intensity on subjective perceptions.

Alpha-Actinin-3 R577X Polymorphism Influences Muscle Damage and Hormonal Responses After a Soccer Game.

Coelho, DB, Pimenta, EM, Rosse, IC, Veneroso, C, Pussieldi, GDA, Becker, LK, De Oliveira, EC, Carvalho, MRS, and Silami-Garcia, E. Alpha-actinin-3 R577X polymorphism influences muscle damage and hormonal responses after a soccer game. J Strength Cond Res 33(10): 2655-2664, 2019-The purpose of this study was to evaluate indicators of muscle damage and hormonal responses after soccer matches and its relation to alpha-actinin-3 (ACTN3) gene expression (XX vs. RR/RX), considering that the R allele produces alpha-actinin-3 and provides greater muscle strength and power. Thirty players (10 XX and 20 RR/RX) younger than 16 years were evaluated in this study. Blood samples were collected immediately before, after, 2, and 4 hours after the games to assess muscle damage (creatine kinase [CK] and alpha-actin) and hormonal responses (interleukin-6 [IL-6], cortisol, and testosterone). Postgame CK was higher as compared to the pregame values in both groups and it was also higher in the RR/RX (p < 0.05) than in the XX. The concentrations of alpha-actin and IL-6 were similar for both groups and did not change over time. Testosterone was increased after the game only in the RR/RX group (p < 0.05). Cortisol concentrations in group RR/RX were higher immediately after the game than before the game, and 2 and 4 hours after the game the concentration decreased (p < 0.05). The RR and RX individuals presented higher markers of muscle microtrauma and hormonal stress, probably because they performed more speed and power actions during the game, which is a self-regulated activity. From the different responses presented by RR/RX and XX genotypes, we conclude that the genotypic profile should be taken into account when planning training workloads and recovery of athletes.

Evidence for a Role of ACTN3 R577X Polymorphism in Football Player's Career Progression.

The aim was to investigate a possible role of the ACTN3 R577X polymorphism in a Brazilian football player's career progression. 2 questions were formulated: 1. Does ACTN3 polymorphism affect the probability of an individual being a professional football player? 2. Does this polymorphism affect the progression of the athlete throughout his career? The study included 353 players from first division Brazilian football clubs in the following categories: under-14 (U-14), U-15, U-17, U-20, and professional (PRO). The control group (CON) was composed of 100 healthy non-athletes. The chi-squared test was used to assess differences between the allele and genotype frequencies. Comparing football categories, the XX genotype was less frequent among professional players than in the U-20 (p<0.05) or the U-15 category (p<0.05). The RX genotype also presented more frequently in the PRO category than the U-14 category (p<0.05). Moreover, a trend towards a higher frequency of the RX genotype and a lower frequency of the XX genotype was observed in the professional category compared to U-20. These results suggest that the genotype in the ACTN3 polymorphism affects the probability of a football player progressing throughout his career and becoming professional, meaning that playing football selects against the ACTN3 XX genotype.

Impact of competitive level and age on the strength and asymmetry of young soccer players / Impacto do nível competitivo e da faixa etária sobre força e assimetria de futebolistas jovens / Impacto del nivel competitivo y franja etaria en la fuerza y asimetría de los futbolistas jóvenes

INTRODUCTION: Muscular strength is an essential capacity for soccer players; however, the profile of this capacity has not yet been clearly established in young players. OBJECTIVE: To compare muscular strength performance in the countermovement jump (CMJ) task and the presence of lower limb asymmetry among soccer athletes of the under 15 (U15), under 17 (U17) and under 20 (U20) categories, and between two teams of different competitive levels. METHODS: One hundred and fifty one young soccer players of three different categories (U15, U17 and U20) from two teams in the Brazilian first and second divisions performed six vertical jumps in a system of two force plates. Ground reaction force (GRF) was used to calculate jump height and to obtain the variables Peak force (FMAX), Impulse (I) and Peak power (PMAX) with regard to body mass and the respective asymmetry values. RESULTS: No differences were found in the performance and asymmetry variable between the categories (Jump height: p=0.23; FMAX: p=0.15; I: p=0.11; PMAX: p=0.32; FMAX asymmetry: p=0.21; I asymmetry: p=0.58; PMAX asymmetry: p=0.58). The first division club had higher muscle strength and asymmetry values than the second division club (Jump height: p<0.01; I: p<0.01; PMAX: p<0.01; FMAX asymmetry: p<0.01; I asymmetry: p<0.01; PMAX asymmetry: p<0.01), except for FMAX (p=0.63). No variable had an interaction effect (club x category). CONCLUSION: As categories progress, jump height, relative I, PMAX and FMAX values and their respective asymmetries are stable. The asymmetry and strength performance of the first division club were higher than that of the second division club, except for FMAX, indicating that the competitive level may influence strength performance and the development of asymmetry. Level of Evidence II; Diagnostic study ­ Investigating a diagnostic test.

INTRODUÇÃO: A força muscular é uma capacidade fundamental para futebolistas; entretanto, o perfil dessa capacidade ainda não está bem estabelecido em jogadores jovens. OBJETIVO: Comparar o desempenho de força muscular na tarefa de salto com contramovimento e a presença de assimetria entre membros inferiores entre atletas de futebol das categorias Sub 15, Sub 17 e Sub 20 e entre dois clubes de níveis competitivos diferentes. MÉTODOS: Cento e cinquenta e um jogadores de futebol jovens de dois clubes da primeira e segunda divisão e de três categorias diferentes (Sub 15, Sub 17 e Sub 20) realizaram seis saltos verticais em um sistema de duas plataformas de força. A partir da força de reação de solo, calculou-se a altura do salto e foram obtidas as variáveis Força máxima (FMÁX), Impulso (I) e Potência máxima (PMÁX) com relação à massa corporal e aos respectivos valores de assimetria. RESULTADOS: Nenhuma variável de desempenho e assimetria foi diferente entre as categorias (Altura do salto: p = 0,23; FMÁX: p = 0,15; I: p = 0,11; PMÁX: p = 0,32; Assimetria de FMÁX: p = 0,21; Assimetria de I: p = 0,58; Assimetria de PMÁX: p = 0,58). O clube de primeira divisão apresentou maior desempenho de força e maior assimetria do que o clube de segunda divisão (Altura do salto: p < 0,01; I: p < 0,01; PMÁX: p < 0,01; Assimetria de FMÁX: p < 0,01; Assimetria de I: p < 0,01; Assimetria de PMÁX: p < 0,01), exceto para FMÁX: (p = 0,63). Nenhuma variável teve efeito de interação (clube x categoria). CONCLUSÃO: À medida que as categorias progridem, a altura do salto e os valores de I, PMÁX e FMÁX relativos e as respectivas assimetrias são estáveis. A assimetria e o desempenho de força do clube da primeira divisão foram maiores do que a do clube da segunda divisão, exceto para FMÁX, mostrando que o nível competitivo pode influenciar o desempenho de força e o desenvolvimento de assimetria. Nível de evidência II; Estudo diagnóstico ­ Investigação de um exame para diagnóstico.

INTRODUCCIÓN: La fuerza muscular es una capacidad fundamental para los futbolistas, sin embargo, aún no está bien establecido el perfil de esa capacidad en jugadores jóvenes. OBJETIVO: Comparar el desempeño de fuerza muscular en la tarea de salto con contramovimiento y la presencia de asimetría entre miembros inferiores de atletas de fútbol de las categorías Sub15, Sub17 y Sub20, y entre dos clubes de fútbol de diferente nivel competitivo. MÉTODO: 151 futbolistas de dos clubes de primera y segunda división y tres categorías diferentes (Sub15, Sub17 y Sub20) realizaron seis saltos verticales en un sistema de plataformas de fuerza. A partir de la fuerza de reacción de suelo, se calculó altura del salto y variables Fuerza máxima (FMÀX), Impulso (I) y Potencia máxima (PMÁX) relativas a la masa corporal y los respectivos valores de asimetría. RESULTADOS: Ninguna variable de rendimiento y asimetría fue diferente entre las categorías (Altura: p=0,23; FMÀX: p=0,15; I: p=0,11; PMÁX: p=0,32; FMÀX: p=0,21; Asimetría de I: p=0,58; Asimetría de PMÁX: p=0,58). El club de la primera división ha presentado mayor rendimiento de fuerza y mayor asimetría que el club de la segunda división (Altura del salto: p<0,01; I: p<0,01; PMÁX: p<0,01; Asimetría de FMÀX: p<0,01, asimetría de I: p<0,01, asimetría de PMÁX: p<0,01), excepto para FMÀX: (p=0,63). Ninguna variable ha presentado efecto de interacción (club x categoría). CONCLUSIÓN: A medida que las categorías progresan, la altura del salto y los valores de IMP, PMAX y FMAX relativos y las respectivas asimetrías se mantienen estables. La asimetría y el desempeño de fuerza del club de la primera división fueron mayores que del club de segunda división, excepto para FMAX, mostrando que el nivel competitivo puede influir en el desempeño de fuerza y en el desarrollo de asimetría. Nivel de evidencia II; Estudio diagnóstico ­ Investigación de un examen para diagnóstico.

Fatal Human Case of Zika and Chikungunya Virus Co-Infection with Prolonged Viremia and Viruria.

Zika virus (ZIKV) infection usually presents as a mild and self-limited illness, but it may be associated with severe outcomes. We describe a case of a 30-year-old man with systemic erythematous lupus and common variable immunodeficiency who became infected with both Zika (ZIKV) and Chikungunya (CHIKV) virus during the 2016 outbreak in Rio de Janeiro, Brazil. The patient presented with intense wrist and right ankle arthritis, and ZIKV RNA and virus particles were detected in synovial tissue, blood and urine, and CHIKV RNA in serum sample, at the time of the diagnosis. During the follow up, ZIKV RNA persisted for 275 days post symptoms onset. The patient evolved with severe arthralgia/arthritis and progressive deterioration of renal function. Fatal outcome occurred after 310 days post ZIKV and CHIKV co-infection onset. The results show the development of severe disease and fatal outcome of ZIKV infection in an immunosuppressed adult. The data suggests a correlation between immunodeficiency and prolonged ZIKV RNA shedding in both blood and urine with progressive disease. The results also indicate a possible role for arbovirus co-infections as risk factors for severe and fatal outcomes from ZIKV infection.

Como ocorreu o processo de término da carreira esportiva de ex-atletas de futebol que disputaram copas do mundo pelo Brasil entre 1966 e 1982? / How did the process of ending the sports career of ex-soccer athletes who played the world cups for Brazil between 1966 and 1982? / ¿Cómo ocurrió el proceso de término de la carrera deportiva de ex atletas de fútbol que disputaron copas del mundo por Brasil entre 1966 y 1982?

O objetivo do estudo foi analisar o término da carreira de ex-atletas de futebol que disputaram Copas do Mundo pelo Brasil entre 1966 e 1982. Foram realizadas entrevistas semiestruturadas com seis ex-atletas. As entrevistas foram analisadas por meio de miniunidades. O conteúdo das entrevistas foi organizado em cinco categorias. Os resultados apontam que os ex-atletas não tiveram acesso a uma estrutura profissional para planejar o término da carreira. Eles não conseguiram conciliar a carreira esportiva com os estudos. Foram citados como motivos para o término da carreira: a saturação psicológica, lesões e idade avançada. Os entrevistados tiveram diversas ocupações no pós-carreira, muitas delas relacionadas com o meio esportivo. A família foi a maior fonte de apoio durante o término da carreira. Conclui-se que as causas da aposentadoria dos entrevistados foram multifatoriais e que os ex-atletas não tiveram uma um planejamento estruturado para o término da carreira esportiva.

This study aimed to analyze the end of the career of former soccer players who competed in World Cup for Brazil between 1966 and 1982. Semi-structured interviews were conducted with six former athletes. The interviews were analyzed by means of meaning-units. The content of the interview was organized into five categories. The results indicate that the former athletes did not have access to a professional structure to plan for after the end of their career. They failed to reconcile their sports career with their studies. The described reasons for the end of careers included psychological saturation, injuries, and old age. The interviewees had several post-career occupations, many of them related to the sports environment. Their families were the greatest source of support during the end of their careers. It is concluded that the causes of the retirement of the interviewees were multifactorial, and that the former athletes did not have a structured plan for after the end of their sports career.

El propósito del estudio fue analizar el término de la carrera de ex atletas de fútbol que disputaron Copas del Mundo por Brasil entre 1966 y 1982. Se realizaron entrevistas semiestructuradas con seis ex atletas. Las entrevistas se analizaron por medio de miniunidades. El contenido de la entrevista se organizó en cinco categorías. Los resultados apuntan que los ex atletas no tuvieron acceso a una estructura profesional para planificar el término de la carrera. Ellos no consiguieron conciliar la carrera deportiva con los estudios. Los motivos mencionados para el término de la carrera fueron: la saturación psicológica, lesiones y edad avanzada. Los entrevistados tuvieron diversas ocupaciones en el periodo posterior a la carrera, muchas de ellas relacionadas con el medio deportivo. La familia fue la mayor fuente de apoyo durante el término de la carrera. Se concluye que las causas de la jubilación de los entrevistados fueron multifactoriales y que los ex atletas no tuvieron una planificación estructurada para el término de la carrera deportiva.

Comparison of two classification criteria of lateral strength asymmetry of the lower limbs in professional soccer players / Comparação de dois critérios de classificação de jogadores de futebol profissional da assimetria lateral de força dos membros inferiores

Abstract Prospective studies have indicated that assessing muscle function by the isokinetic test in the preseason is able to identify muscle strength variables that can be associated with injuries in the hamstring muscles of professional soccer players. However, the results are conflicting and could be related to the different cutoff points for the categorization of athletes in asymmetric or symmetric. Based on these conflicting results, the present study aims to: i) Identify the cutoff points of lateral asymmetry (LA) of the 95% confidence interval for the peak torque in soccer players, evaluated by the isokinetic test, taking into consideration the mean population value; ii) Compare LA between 15% cutoff point proposed in literature and the upper limit of 95% confidence interval (UP) of the study population. Sixty-four professional soccer players performed five knee flexion and extension repetitions at rate of 60°/s, with a one-minute interval. To determine the cutoff point for strength asymmetries, the UP was used, and for the purpose of diagnostic concordance of information among the different values, the McNemar's χ2 test was applied. The proportion between symmetric and asymmetric athletes was not different from the 15% cutoff point and the general population, both for knee flexors (χ2 = 0.5, p = 0.250) and the knee extensors (χ2 = 2.0, p = 0.125). It was concluded that the cutoff point of 15% referenced in literature for the general population provides similar diagnostic information for the classification of professional soccer players.

Resumo Estudos prospectivos têm indicado que avaliar a função muscular pelo teste isocinético na pré-temporada é capaz de identificar variáveis da força muscular que se associam a lesões nos músculos posteriores da coxa em jogadores de futebol, entretanto, os resultados são conflitantes e podem estar relacionados aos diferentes pontos de corte para categorização do atleta em assimétrico. Diante de resultados antagônicos, o presente estudo objetivou: i) Identificar o ponto de corte da assimetria lateral (AL) do intervalo de confiança de 95% para o pico de torque em jogadores de futebol, avaliado no teste isocinético, levando em consideração a média populacional; ii) Comparar a AL entre o valor de corte de 15%, proposto pela literatura, e o limite superior do intervalo de confiança de 95% (LS) da população estudada. 64 jogadores de futebol profissional realizaram cinco repetições máximas de flexão e extensão do joelho na velocidade de 60°/s com intervalo de um minuto entre repetições. Para determinação do ponto de corte da AL, utilizou-se o LS e para a concordância da informação diagnóstica entre os diferentes valores de corte foi aplicado o teste χ2 de McNemar. A proporção entre simétricos e assimétricos não foi diferente entre os valores de corte de 15% e o populacional, tanto para os flexores do joelho (χ2 = 0.5; p = 0.250) quanto para os extensores do joelho (χ2 = 2.0; p = 0.125). Conclui-se que, o ponto de corte populacional proporciona uma classificação dos atletas de futebol profissional similar ao valor de 15% referenciado na literatura.

Effect of a professional soccer match in skin temperature of the lower limbs: a case study.

The purpose of this study is to analyze the relationship between lower limbs skin temperature (Tsk) recorded by infrared thermography and creatine kinase (CK) response following an official soccer game. A professional soccer player, from the first division Brazilian League, defender, 27 years and 183 cm of height was analyzed. Measures of Tsk with a thermal camera and blood CK concentrations were obtained 24 hr before (24H-PRE), 24 (24H-POST) and 48 hr (48H-POST) after an official match. The results showed that CK values were 193 U/L 24 hr before, rising to 1,083 U/L 24 hr after the game and 414 U/L 48 hr after the game. Anterior thigh Tsk was 31.5°C, 33.8°C, and 31.8°C in the moments 24H-PRE, 24H-POST, and 48H-POST, respectively. Similarly, anterior leg presented temperature of 31.2°C, 33.3°C, and 31.5°C at the same moments. Qualitative analysis of the thermograms showed that whole lower limbs are much warmer 24 hr after the match and certain areas as the right anterior thigh, the left anterior leg, both anterior ankles, and both posterior thighs have not fully recovered their initial Tsk 48 hr after the match. The results of this study indicate that participation in a professional soccer match can lead to significant increases in Tsk values measured by IRT 24 hr after the match. Considering this variable as an indicator of muscular damage, it could help in the process of training control, being part of an injury prevention program in professional soccer clubs.

Zika virus shedding in human milk during lactation: an unlikely source of infection?

Zika virus (ZIKV) transmission through non-mosquito-dependent routes has become increasingly important since reports of sexual transmission. Breastfeeding is a potential means of ZIKV transmission, but data on this remain limited. The cases of four mothers with laboratory-proven infections are reported. No disease evolved in three of the breastfed babies despite detectable maternal viremia and viruria, the presence of viral RNA shedding, and the isolation of infective particles in one milk sample. Fever and rash in one infant of a ZIKV-infected mother proved to be related to chikungunya virus infection. The results suggest that the presence of infective particles in breast milk may not be sufficient for the efficient perinatal transmission of ZIKV.

Validity of inner canthus temperature recorded by infrared thermography as a non-invasive surrogate measure for core temperature at rest, during exercise and recovery.

Research into obtaining a fast, valid, reliable and non-invasive measure of core temperature is of interest in many disciplinary fields. Occupational and sports medicine research has attempted to determine a non-invasive proxy for core temperature particularly when access to participants is limited and thermal safety is of a concern due to protective encapsulating clothing, hot ambient environments and/or high endogenous heat production during athletic competition. This investigation aimed to determine the validity of inner canthus of the eye temperature (TEC) as an alternate non-invasive measure of intestinal core temperature (TC) during rest, exercise and post-exercise conditions. Twelve physically active males rested for 30min prior to exercise, performed 60min of aerobic exercise at 60% V̇O2max and passively recovered a further 60min post-exercise. TEC and TC were measured at 5min intervals during each condition. Mean differences between TEC and TC were 0.61°C during pre-exercise, -1.78°C during exercise and -1.00°C during post-exercise. The reliability between the methods was low in the pre-exercise (ICC=0.49 [-0.09 to 0.82]), exercise (ICC=-0.14 [-0.65 to 0.44]) and post-exercise (ICC=-0.25 [-0.70 to 0.35]) conditions. In conclusion, poor agreement was observed between the TEC values measured through IRT and TC measured through a gastrointestinal telemetry pill. Therefore, TEC is not a valid substitute measurement to gastrointestinal telemetry pill in sports and exercise science settings.